- PEN PAD DRIVER MICROMANAGER INSTALL
- PEN PAD DRIVER MICROMANAGER SERIAL
- PEN PAD DRIVER MICROMANAGER SOFTWARE
PEN PAD DRIVER MICROMANAGER SERIAL
But things seem fairly unstable (much more stable with a good USB serial adapter, but auto-detection is still problematic). Without this is place, the X/Y stage will not move on my unit, the Z stage and filter wheel will however happily work. Take care when creating/installing this as shorting the wrong pins can damage the X/Y card (see “Cards” below). The following image shows a 1K resistor between these two pins, and when connected to the “LIN ENC” socket will allow the X/Y stage to operate. This seems to provide a hardware lockout on the X/Y stage when the door is open. The only pins connected on the genome analyzer are pins 5 and 8. In the genome analyzer the socket labeled “LIN ENC” on the X/Y card is as I understand it connected to the door sensor. This doesn’t take long and insures that all the necessary libraries are installed.
PEN PAD DRIVER MICROMANAGER INSTALL
My route to getting this working was to create a Windows 7 VM, then install Visual Studio 6. The various runtime library either don’t work, or don’t include all the necessary components for Windows 10.
PEN PAD DRIVER MICROMANAGER SOFTWARE
The software is somewhat troublesome to get working on modern systems and appears to have been written in Visual (Basic?) 6. This software is a single exe called “TLX4000s.exe”, available from this excellent blog post on the GA2 ASI stages (or local copy). I found that initial setup with the ASI software is most reliable.
Micro manager is supposed to support the LX4000, but depending on the version you have you may have issues. However commands need to be prefix to address the required stage as follows:
The LX4000 command set seems to be similar to the MS2000. However, the ASI TLX4000s.exe application will happily communicate with the controller and control everything except the X/Y stage without further modification (see notes on the X/Y stage below). I had issues getting the device to respond here. However after a few seconds the device will start replying with “BELL”s (or possibly just in hex). While the instrument is initializing it’s possible to enter serial commands such as “1H I Z”, which will return information regarding the Z axis. The general command set seems to follow the MS-2000 protocol. This indicates that only one filter wheel is installed, and is not an error. It may also report “MOTOR 1 NOT RESPONDING” or similar. When powered up the serial will output “F” or “FX”. Baud rate is 115200 (8N1) on the “Z/F” card. The USB-serial adapter should be attached to the usually included “Reverser” (I believe a null modem converter).
-31.jpg "pen pad driver micromanager")
Probably a (real) FTDI device would work well too. The Edgeport 8port serial card supplied with the Genome analyzer works well.
I therefore recommend getting a better USB serial adapter. A number of issues I had with getting the LX4000 to work with micro manager were caused by the device only registering some of the commands the CH340 was sending. Many CH340 RS232 converters work quite poorly, seemly mostly on the transmit side. The filter wheel can be controlled manually by pressing the “NEXT” button or via the “Z/F” serial interface.Ī CH340 based USB serial converter works well with the LX4000. The FW-1000 card has a port labelled “RS-232” on my instrument this is not physically connected to anything. Commands for the other cards are routed over the internal backplane. CommunicationĪll 3 cards have ports that look like RS232 ports, only the port on the “Z/F” card can be used to communicate with the device. These PCBs are labelled “Two Axis Board” on my device one is “REV. The “Z/F” and “X/Y” cards are essentially the same design. The FW-1000 (filter wheel), a “Z/F” card (Objective, Z-axis), and a “X/Y” card. This post contains my notes on using this controller. The controller is essentially a chassis with a backplane providing power and common bus to expansion cards. It’s a custom stage controller made by ASI somewhat similar to their ASITiger controllers. The LX-4000 is the stage controller used in the Illumina Genome Analyzer 2.
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